Mechanism of inhibition of platelet activation by tanshinone type ⅡA though G protein signal pathway / 国际检验医学杂志

Objective To discuss the mechanism of inhibition of platelet activation by tanshinone type ⅡA(TanⅡA) through G protein signal pathway.Methods Methylthiazolyldiphenyl-tetrazolium bromide(MTT) test was used to determine the optimum effective concentration and optimal time of thrombin and TanⅡA on platelet.Northern blot and Western blot were used to detect the transcription and expression levels of G protein and related signal molecules,including protease activated receptors(PARs),P2Y1 and P2Y12 receptors,α2A-adrenergic receptor and thromboxane A2(TXA2) receptor,in control group,thrombin treated group and TanⅡA treated group,and the platelet aggregation rate was also detected.Results Platelet aggregation rate,and the transcription and expression levels of G protein and related molecules in thrombin treated group were higher than control group(P<0.05).The transcription and expression levels of G protein and related molecules in different concentrations of TanⅡA treated groups were lower than thrombin treated group(P<0.05).Conclusion TanⅡA could inhibit the activation of platelet by inhibiting the transcription and expression of G protein and the related molecules.

Three misunderstandings in talent introduction in the hospital / 中华医院管理杂志

The paper points out that at present the phenomenon of "three emphases and three contempts" is rather prevalent in talent introduction, viz. emphasis on talents from the outside and contempt for talents from the inside, emphasis on individuals and contempt for teams, and emphasis on introduction and contempt for use. Countermeasures for improvement are proposed with regard to the causes of the three misunderstandings and harmful consequences.